Figure 4.

Effect of inhibiting CK2 activity on the E7–pRB interaction. HEK293 cells were transfected with the empty pCMV vector or with pCMV:FLAG-HA-HPV-16 E7. The cell lysates were immunoprecipitated using an anti-HA antibody immobilized on agarose beads. (A). The immunoprecipitated complexes were then washed with lysis buffer and analyzed by western blot for pRB and total E7. The panel shows the protein inputs and the results of the immunoprecipitation. (B). Quantification of the levels of immunoprecipitated pRB with respect to the levels of E7 in the presence of CK2 inhibitors. Data are shown as means ± SD, n = 3. Statistically significant differences between vehicle and drug treatment are indicated with *** p < 0.001, determined using one-way ANOVA followed by Dunnett’s post-test.