Figure 7.
Resveratrol reduced nucleocapsid expression of MERS-CoV. Vero E6 cells were infected by MERS-CoV (M.O.I. 0.1) and treated with resveratrol for 24 h followed by 4% paraformaldehyde fixation for immunofluorescent assays. (a) Nucleocapsid expressions were examined with confocal microscope at 680× magnification. DAPI was used for nucleus staining. (b) Intracellular staining of MERS nucleocapsid expressions were visualized by Odyssey® CLx Imaging system. (c) Quantification results of fluorescent intensities of MERS nucleocapsid proteins were determined by Odyssey® CLx Imaging software. (Reproduced with permission from Ref. [115]).