Figure 5.

Analyses of CD8⁺ T cell memory formation. T cell memory population was investigated in WT or NAC1^−/− mice after VACV challenge. The mice were sacrificed on day 35. The spleen and LNs were dissected, smashed, and stained before flow cytometry. (A) Memory CD8⁺ T cells (CD8⁺ CD44⁺). (B) VACV-specific memory CD8⁺ T cells (CD8⁺ CD44⁺ B8R⁺), gating on memory CD8⁺ T cells. (C) Tissue-resident memory (CD44^hiCD69^hi CD197^low), central memory (CD44^hiCD69^low CD197^hi) and effector memory (CD44^hiCD69^low CD197^low) T cells, gating on VACV-specific memory CD8⁺ T cells. The plotting data shown are representative of three identical experiments (n = 5).