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. 2022 Aug 4;14(8):1713. doi: 10.3390/v14081713

Figure 6.

Figure 6

Enhanced CD8+ T cell memory formation in NAC1−/− mice. Quantification for T cell memory population after 35 days. The mice were sacrificed on day 35. The spleen and lymph nodes were dissected and smashed. The total live cell number for each mouse was calculated with trypan blue staining through a Bio-Rad cell counter. T cell surface markers were stained before flow cytometry. (A) The VACV-specific memory CD8+ T cell (CD8+ CD44+ B8R+) number for each mouse had been calculated and compared between WT and NAC1−/− groups. (B) The VACV-specific memory CD8+ T cell (CD8+ CD44+ B8R+) frequency for each mouse was collected and analyzed. The p-value is 0.040. (C) Tissue-resident memory (CD44hiCD69hi CD197low) T cell frequency had been analyzed between WT and NAC1−/− groups. (*, p < 0.05; ns, p > 0.05).