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Chromatographic separation of LMW succinoglycan on Sephadex DEAE A-25. The column was eluted with a linear gradient of KCl in a MOPS buffer (10 mM; pH 7). Fractions (4 ml) were collected, and carbohydrates were assayed by anthrone-sulfuric acid method. (A) Monomers (elution, 400 ml of 5 to 250 mM KCl); (B) Dimers (elution, 600 ml of 5 to 400 mM KCl); (C) Trimers of succinoglycan (elution, 600 ml of 5 to 500 mM KCl).
