TABLE 3.
Sample | % Compositional subunitsb
|
||
---|---|---|---|
S0 | S1 | S2 | |
D1 | 50 | 50 | 0 |
D2 | 20 | 61 | 19 |
D3 | 0 | 50 | 50 |
T1 | 69 | 31 | 0 |
T2 | 43 | 50 | 7 |
T3 | 25 | 56 | 19 |
T4 | 5 | 53 | 42 |
T5 | 3 | 34 | 63 |
Samples D1 to D3 and T1 to T5 were degraded to the compositional octasaccharide subunits by the succinoglycan depolymerase from Cytophaga. The products were analyzed and quantified by Dionex HPAEC.
S0, S1, and S2 represent the octasaccharide subunits containing 0, 1, and 2 succinyl groups, respectively. The percentages were calibrated from standard samples (M1, M2, and M3). The molar responses in PAD detection under the HPAEC-PAD condition for S0, S1, and S2 are 1:1:0.66. The reason for the lower response for S2 is not clear.