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. 1999 Nov;181(21):6788–6796. doi: 10.1128/jb.181.21.6788-6796.1999

TABLE 3.

HPAEC determination of the succinyl distributions within the oligomersa

Sample % Compositional subunitsb
S0 S1 S2
D1 50 50 0
D2 20 61 19
D3 0 50 50
T1 69 31 0
T2 43 50 7
T3 25 56 19
T4 5 53 42
T5 3 34 63
a

Samples D1 to D3 and T1 to T5 were degraded to the compositional octasaccharide subunits by the succinoglycan depolymerase from Cytophaga. The products were analyzed and quantified by Dionex HPAEC. 

b

S0, S1, and S2 represent the octasaccharide subunits containing 0, 1, and 2 succinyl groups, respectively. The percentages were calibrated from standard samples (M1, M2, and M3). The molar responses in PAD detection under the HPAEC-PAD condition for S0, S1, and S2 are 1:1:0.66. The reason for the lower response for S2 is not clear.