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. 2022 Aug 13;15(8):997. doi: 10.3390/ph15080997

Figure 6.

Figure 6

Trapping experiments were conducted in whole-cell patch-clamp electrophysiology at −70 mV holding potential. Trapping experiments were designed to measure trapped block (BT) of 10 µM REL-1017 or 1 µM (±)-ketamine that is % ratio between residual block (BR) after extensive (85 s) cell wash with glycine alone and initial test item block (B). Test item application protocol diagram (top) and sample traces (bottom) of trapping experiments with 10 µM REL-1017-treated cell (left) or 1 µM (±)-ketamine-treated cell (right) are shown. I1st and I2nd were the peak currents measured within 1 s after onset of the first and second 10 µM/10µM L-glutamate/glycine addition, respectively. Results from trapping experiments are reported in Table 6. s is seconds.