Table 4.

Onset kinetic parameters.

	Tau on (s)	I1 (% Current)	Cell Number
10 µM REL-1017	4.7 ± 0.21	20.4%	11
1 µM (±)-ketamine	4.7 ± 0.14	28.7%	10
10 µM (±)-ketamine	0.99 ± 0.050 ****	3.6%	4

Experiments were conducted in whole-cell patch-clamp electrophysiology at a holding potential of −70 mV. Onset kinetic constant (tau on) of an NMDAR antagonist represents the time required for the test item to reach approximately 63.2% of its current blocking effect. Tau on (s) is reported as mean ± SEM, while I₁ is the % current measured at the end of test item addition and normalized with respect to I₀ current, the current value before test item addition, which is set equal to 100%. Data passing normality (Shapiro–Wilk) test were analyzed with one-way ANOVA followed by Tukey’s test. **** is p < 0.0001. s is seconds.