Table 5.

Offset kinetic parameters.

	Tau off (s)	I2 (% Current)	Cell Number
10 µM REL-1017	17.7 ± 1.0	98.5%	11
1 µM (±)-ketamine	15.2 ± 0.63	95.5%	10
10 µM (±)-ketamine	17.2 ± 3.0	102.9%	4

Experiments were conducted in whole-cell patch-clamp electrophysiology at a holding potential of −70 mV. Offset kinetic constant (tau off) of an NMDAR antagonist represents the time required for the removal of approximately 63.2% of the current blocking effect of the test molecules, in continuous perfusion with a buffer containing the agonist L-glutamate but not the test item. Tau off (s) is reported as mean ± SEM, while I₂ (% current) is measured at the end of test item removal and normalized with respect to I₀ current, the current value before test item addition, which is set equal to 100%. Data passing normality (Shapiro–Wilk) test were analyzed with one-way ANOVA followed by Tukey’s test. Tau off values results were not significantly different. s is seconds.