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. 2022 Mar 4;3(3):240–263. doi: 10.1158/2643-3230.BCD-21-0163

Figure 3.

Figure 3. Human CD34 cells expressing ZNF384 FO exhibit immature myeloid cell expansion. A, CFU of CD34 human cord blood cells expressing empty vector, wild-type, or FO. Columns show means of two replicates ± SD. Colonies were scored based on morphology; macrophage (CFU-M), granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM), granulocyte, macrophage (CFU-GM), burst forming unit-erythroid (BFU-E), or erythroid (CFU-E). B, Cells harvested from CFU were subjected to flow cytometry. FO-expressing cells have an expansion of CD45- and CD33-positive cells compared with controls. FO samples also have CD19, CD33 double-positive cells. C, Cloning efficiency and lineage outcomes of single cells from indicated genotypes. Data in B and C represent cells pooled from replicates for analysis.

Human CD34 cells expressing ZNF384 FO exhibit immature myeloid cell expansion. A, CFU of CD34 human cord blood cells expressing empty vector, wild-type, or FO. Columns show means of two replicates ± SD. Colonies were scored based on morphology; macrophage (CFU-M), granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM), granulocyte, macrophage (CFU-GM), burst forming unit-erythroid (BFU-E), or erythroid (CFU-E). B, Cells harvested from CFU were subjected to flow cytometry. FO-expressing cells have an expansion of CD45- and CD33-positive cells compared with controls. FO samples also have CD19, CD33 double-positive cells. C, Cloning efficiency and lineage outcomes of single cells from indicated genotypes. Data in B and C represent cells pooled from replicates for analysis.