Table 6.

Preparations of the PCR reaction.

PCR Reaction Mixture	Reaction Volume
	Conventional PCR	Multiplex PCR
2x Taq Master Mix	5 μL	20 μL
PCR grade water	2 μL	3 μL
Forward primer	1 μL	6 μL
Reverse primer	1 μL	6 μL
Template DNA	1 μL	5 μL
Total	10 μL	40 μL