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Analyses of cytokine mRNA expression in mice using qPCR. Bar graphs represent relative IFNG, IL-1a, IL-4, IL-6, and TNF mRNA expression levels after normalization to the GAPDH gene in naive and immunized mice with later Ehrlichia canis challenge group (50 µg rGP19, 100 µg rGP19, and adjuvant). Data are represented as mean ± SE (n = 5 each treatment), one-way ANOVA, * p < 0.05, ** p < 0.01.
