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. 2022 Aug 10;14(16):3260. doi: 10.3390/polym14163260

Figure 5.

Figure 5

In vitro osteogenesis properties of NG/nHA/COL. BMSCs were cultured using nHA/COL and NG/nHA/COL culture media extracts. (a) ALP staining images of control, nHA/COL, and NG/nHA/COL groups after 7 d of osteogenic induction (scale bar: 200 µm). (b) ARS staining images of control, nHA/COL, and NG/nHA/COL groups after 21 d of osteogenic induction (scale bar: 100 µm). (c) ALP activity of three groups (n = 3). (d) Quantitative results of ARS staining of three groups (n = 3). (e) The osteogenic-related genes expression, including BMP-2, OPN, OCN, and Runx-2 (n = 3).