FIG. 2.

In vitro transcription with reconstituted RNA polymerases (50 nM). Experimental conditions were as described previously (5). Values are relative to the ratio of alkA/lacUV5 transcripts for Eς⁷⁰. When necessary, the Ada protein was present at 0.2 μM. Data are an average from the results of three experiments. Standard deviation was less than 15%.