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Gel mobility shift assays with dsDNA fragments containing aadA1 attC (left) or the attI site (right). Lanes 1, control dsDNA; lanes 2, dsDNA plus E. coli C41 control extract; lanes 3, dsDNA plus pure IntI1-COOH; lanes 4, dsDNA plus purified IntI1. F, free dsDNA; B, DNA-protein complex.
