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. 2022 Aug 18;14(16):3395. doi: 10.3390/nu14163395

Figure 4.

Figure 4

SLC20A1 is indispensable for Pi metabolism and survival of HSCs post IR. (A) Relative mRNA expression of Slc20a1 in BM LSK cells of mice post IR (n = 3). (B) Representative flow cytometric analysis and fold change of mean fluorescence intensity (MFI) of SLC20A1 protein in BM HSCs of mice at 3 dpi (n = 6). (C) Fold change of SLC20A1 MFI in BM HSCs of mice with or without Klotho treatment at 3 dpi (n = 5). (D) Fold change of SLC20A1 MFI in BM HSCs of WT and Kl+/− mice at 3 dpi (n = 5). (E) Scheme for the creation of mouse model of irradiation-induced myelosuppression using Slc20a1+/− mice and HSC analysis. (F) Pi content in BM LSK cells of WT and Slc20a1+/− mice post IR (n = 6). (G) Relative HSC numbers in the BM of WT and Slc20a1+/− mice post IR compared to those at 0 dpi (n = 6). (H) Representative flow cytometric analysis and quantification of apoptotic HSCs in the BM of WT and Slc20a1+/− mice at 3 dpi (n = 6). (I–K) WBC, RBC, and platelet counts in PB of WT and Slc20a1+/− mice post IR (n = 6). Data are mean ± SD. * p < 0.05, ** p < 0.01. Two-tailed unpaired Student’s t-test.