Figure 9.
Overexpressed ZFAS1 reduced brain tissue infarction and apoptosis caused by MCAO, while miR-421-3p mimic had the opposite effect. (A) The effects of overexpressed ZFAS1 or miR-421-3p on the expression of ZFAS1 in the brain tissues of MCAO-modeled mice were tested by qRT-PCR. GAPDH was an internal reference. (B) The effects of overexpressed ZFAS1 or miR-421-3p on the expression of miR-421-3p in the brain tissues of MCAO-modeled mice were detected by qRT-PCR. GAPDH was an internal reference. (C-D) The effects of overexpressed ZFAS1 or miR-421-3p on the infarct area in the brain tissues of MCAO-modeled mice were observed and analyzed by TTC staining. (E-F) The effects of overexpressed ZFAS1 or miR-421-3p on cell apoptosis in the brain tissues of MCAO-modeled mice were observed and analyzed by TUNEL staining. n = 5. ++p < 0.01, +++p < 0.001 vs Sham; *p < 0.05, **p < 0.01, ***p < 0.001 vs MCAO+ZFAS1+ Scramble; ##p < 0.01, ###p < 0.001 MCAO+ZFAS1+ Scramble; &p < 0.05, &&p < 0.01, &&&p < 0.001 vs MCAO+ZFAS1+ miR421-3p Agomir.