Figure 3.

Thiazolyl-pyrazoline derivatives downregulated p-EGFR and p-VEGFR-2 protein levels (A) Western blotting analysis of the expression of p-EGFR, EGFR, p-VEGFR-2 and VEGFR-2 in A549 cells treated with 10b or 10d and untreated cells used as a negative control. β-actin served as a loading control. (B) The relative protein expression level of p-EGFR normalised with total EGFR was quantified by the Image J software (C) The relative protein expression level of p-VEGFR-2 normalised with total VEGFR-2. The values are expressed as the mean ± SEM based on three different experiments. ***p < 0.001 indicate a significant difference compared with control.