Figure 7.

Apoptotic effect of compounds 10b and 10d on A549 cells. (A) Representative cytograms of apoptotic A549 cells stimulated with 0 (control), 2.5 or 5 μM 10b or 10d, for 24 h. The lower-right (annexin V + PI- cells) and the upper-right (annexin V + PI + cells) quadrants show early and late apoptotic cells, and the lower-left (annexin V-PI- cells) and the upper-left (annexin V-PI- cells) quadrants represent viable and necrotic cells, respectively. (B) Quantification of annexin V-positive apoptotic cells upon stimulation with 10b or 10d. The values are the means ± SEM of three different experiments. *p < 0.05 and *p < 0.01 indicate significant differences compared with control.