Table 1.
Overview of results for the validation parameters: recovery, repeatability, reproducibility and measurement uncertainty are calculated for each microcystin congener (MC), Nodularin (NOD) and the sum at each independent concentration level and on average.
| Toxins | Spiked Concentration (µg kg−1) | Recovery (%) | Repeatability (%) | Reproducibility (%) | Measurement Uncertainty (%) | Average S/N * LOD (22.5 µg kg−1) | Average S/N LOQ (50 µg kg−1) | R2 ** |
|---|---|---|---|---|---|---|---|---|
| MC-RR | 50 | 80.00 | 4.20 | 13.26 | 26.53 | 224.16 | 445.87 | 0.998 |
| 400 | 76.00 | 8.87 | 9.46 | 18.91 | ||||
| 800 | 84.00 | 1.64 | 14.02 | 28.05 | ||||
| 1200 | 83.00 | 1.10 | 11.68 | 23.36 | ||||
| Average | 80.75 | 3.95 | 12.11 | 24.21 | ||||
| NOD | 50 | 95.00 | 3.30 | 7.73 | 15.46 | 220.22 | 328.44 | 0.998 |
| 400 | 86.00 | 8.94 | 15.46 | 30.92 | ||||
| 800 | 89.00 | 2.50 | 15.55 | 31.11 | ||||
| 1200 | 89.00 | 0.91 | 13.19 | 26.39 | ||||
| Average | 89.75 | 3.91 | 12.99 | 25.97 | ||||
| MC-LA | 50 | 102.00 | 2.79 | 10.52 | 21.03 | 35.10 | 74.09 | 0.998 |
| 400 | 95.00 | 8.47 | 13.60 | 27.20 | ||||
| 800 | 99.00 | 2.06 | 6.95 | 13.90 | ||||
| 1200 | 99.00 | 1.17 | 4.33 | 8.65 | ||||
| Average | 98.75 | 3.62 | 8.85 | 17.69 | ||||
| MC-LF | 50 | 102.00 | 3.72 | 8.06 | 16.11 | 34.01 | 78.61 | 0.999 |
| 400 | 99.00 | 9.37 | 10.96 | 21.91 | ||||
| 800 | 103.00 | 1.40 | 14.05 | 28.10 | ||||
| 1200 | 103.00 | 1.48 | 14.33 | 28.67 | ||||
| Average | 101.75 | 3.99 | 11.85 | 23.70 | ||||
| MC-LR | 50 | 92.00 | 5.61 | 7.06 | 14.12 | 73.46 | 142.57 | 0.998 |
| 400 | 79.00 | 8.86 | 18.74 | 37.48 | ||||
| 800 | 82.00 | 2.54 | 13.72 | 27.45 | ||||
| 1200 | 82.00 | 1.88 | 7.04 | 14.08 | ||||
| Average | 83.75 | 4.72 | 11.64 | 23.28 | ||||
| MC-LY | 50 | 102.00 | 3.50 | 8.01 | 16.02 | 25.53 | 49.62 | 0.998 |
| 400 | 94.00 | 9.07 | 15.53 | 31.06 | ||||
| 800 | 96.00 | 1.21 | 8.80 | 17.61 | ||||
| 1200 | 96.00 | 0.75 | 3.13 | 6.26 | ||||
| Average | 97.00 | 3.63 | 8.87 | 17.74 | ||||
| MC-LW | 50 | 103.00 | 3.77 | 3.85 | 7.70 | 23.62 | 50.09 | 0.998 |
| 400 | 100.00 | 8.50 | 11.25 | 22.50 | ||||
| 800 | 104.00 | 2.24 | 15.13 | 30.26 | ||||
| 1200 | 103.00 | 1.64 | 14.54 | 29.07 | ||||
| Average | 102.50 | 4.04 | 11.19 | 22.38 | ||||
| MC-YR | 50 | 86.00 | 3.31 | 9.85 | 19.70 | 33.86 | 68.87 | 0.997 |
| 400 | 73.00 | 10.21 | 16.81 | 33.61 | ||||
| 800 | 75.00 | 1.85 | 16.21 | 32.42 | ||||
| 1200 | 75.00 | 1.93 | 12.24 | 24.47 | ||||
| Average | 77.25 | 4.33 | 13.78 | 27.55 | ||||
| MC-WR | 50 | 90.00 | 3.24 | 16.00 | 32.00 | 29.17 | 79.90 | 0.999 |
| 400 | 69.00 | 9.59 | 15.89 | 31.78 | ||||
| 800 | 69.00 | 2.04 | 13.88 | 27.66 | ||||
| 1200 | 69.00 | 2.01 | 9.65 | 19.30 | ||||
| Average | 74.25 | 4.22 | 13.86 | 27.69 | ||||
| Sum | 450 | 95.00 | 1.90 | 4.98 | 9.95 | N.A. | N.A. | N.A. |
| 3600 | 86.00 | 8.98 | 13.17 | 26.34 | ||||
| 7200 | 89.00 | 1.62 | 11.98 | 34.96 | ||||
| 10800 | 89.00 | 0.83 | 9.48 | 30.22 | ||||
| Average | 89.75 | 3.33 | 9.90 | 25.37 |
* Signal to Noise (S/N) is assessed as parameter for the limit of quantification (LOQ). LOQ is assessed at the lowest spiked concentration with an acceptable threshold of 10. S/N is assessed for the limit of detection (LOD) at the lowest concentration in the calibration curve with an acceptable threshold of 3. ** Linearity of the calibration curve for each toxin is determined by R2.The LOD and LOQ are assessed for each toxin separately. LOD and LOQ is not calculated for the sum of the concentrations for all toxins and is therefore not applicable (N.A.).