Table 2.
Analysis of viral viability in cell culture following treatment with disinfectants.
| Samples Collected after Washing with Commercial Disinfectant Products | Days Post Infection | |||||||
|---|---|---|---|---|---|---|---|---|
| 1st Day | Ct Value | 5th Day | Ct Value | 7th Day | Ct Value | 9th Day | Ct Value | |
| A.1 R 1st VS1 | NO | NEG | LC | NEG | MLC | NEG | MLC | NEG |
| A.2 R 1st VS1 | NO | NEG | LC | NEG | LC | NEG | LC | NEG |
| A.1 R 2nd VS1 | NO | NEG | MLC | NEG | CTEcc | NEG | __ | X |
| A.2 R 2nd VS1 | NO | NEG | MLC | NEG | CTEcc | NEG | __ | X |
| A.1 R 3rd VS1 | NO | NEG | LC | NEG | MLC | NEG | MLC | NEG |
| A.2 R 3rd VS1 | NO | NEG | LC | NEG | MLC | NEG | MLC | NEG |
| A.1 R 1st VS2 | NO | NEG | NO | NEG | MLC | NEG | MLC | NEG |
| A.2 R 1st VS2 | NO | NEG | NO | NEG | MLC | NEG | MLC | NEG |
| A.1 R 2nd VS2 | NO | NEG | NO | NEG | MLC | NEG | MLC | 36.87 |
| A.2 R 2nd VS2 | NO | NEG | NO | NEG | MLC | NEG | MLC | NEG |
| A.1 R 3rd VS2 | NO | NEG | NO | NEG | LC | NEG | LC | NEG |
| A.2 R 3rd VS2 | NO | NEG | NO | NEG | MLC | NEG | MLC | NEG |
| Mock w.1 R 1st VS1 | NO | 33.76 | CPE | 35.46 | CPEcc | 34.69 | __ | X |
| Mock w.2 R 1st VS1 | NO | 34.12 | CPE | NEG | CPEcc | 35.00 | __ | X |
| Mock w.1 R 2nd VS1 | NO | 35.63 | CPE | 38.58 | CPEcc | 3.57 | __ | X |
| Mock w.2 R 2nd VS1 | NO | 34.42 | CPE | 35.55 | CPEcc | 3.49 | __ | X |
| Mock w.1 R 3rd VS1 | NO | 35.06 | CPE | NEG | CPEcc | 36.00 | __ | X |
| Mock w.2 R 3rd VS1 | NO | NEG | CPE | NEG | CPEcc | NEG | __ | X |
| Mock w.1 R 1st VS2 | NO | NEG | CPE | NEG | MLC | 15.78 | CPEcc | 11.47 |
| Mock w.2 R 1st VS2 | NO | 28.20 | CPEcc | 10.01 | __ | X | __ | X |
| Mock w.1 R 2nd VS2 | NO | NEG | CPE | NEG | MLC | NEG | MLC | NEG |
| Mock w.2 R 2nd VS2 | NO | 30.60 | CPEcc | 10.00 | __ | X | __ | X |
| Mock w.1 R 3rd VS2 | NO | __ | CPE | NEG | MLC | 20.43 | CPEcc | 13.77 |
| Mock w.2 R 3rd VS2 | NO | 30.01 | CPEcc | 9.76 | __ | X | __ | X |
| SP.1 R 3rd VS1 | NO | __ | LC | NEG | MLC | NEG | CPEcc | 36.12 |
| SP.2 R 3rd VS1 | NO | __ | NO | NEG | MLC | NEG | CEcc | 27.42 |
| SP.1 R 3rd VS2 | NO | __ | LC | NEG | MLC | NEG | MLC | NEG |
| SP.2 R 3rd VS2 | NO | __ | MLC | NEG | MLC | NEG | MLC | NEG |
| SH.1 R 3rd VS2 | NO | NEG | LC | NEG | LC | NEG | LC | NEG |
| SH.2 R 3rd VS2 | NO | 35.29 | LC | NEG | LC | NEG | LC | NEG |
| SP + L30.1 R 3rd VS1 | NO | 35.30 | LC | NEG | LC | NEG | LC | NEG |
| SP + L30.2 R 3rd VS1 | NO | NEG | MLC | NEG | MLC | NEG | MLC | NEG |
| SP + L30.1 R 3rd VS2 | NO | NEG | MLC | NEG | MLC | NEG | MLC | NEG |
| SP + L30.2 R 3rd VS2 | NO | NEG | MLC | NEG | MLC | NEG | MLC | NEG |
NO, no effect; CPE, cytopathic effect; CPEcc, cytopathic effect and collected cells; CTEcc, cytotoxic effect and collected cells; LC, loose cells; MLC, many loose cells; NEG, negative; POS, positive. Commercial disinfectant products; SP, soap; SP + L, soap and Lysoform®; SP + SH, soap, and sodium hypochlorite; SH, sodium hypochlorite; A, alcohol; and MW, wash mock.