Table 2.

The PCR protocol for amplification of the V3 hypervariable region of the 16S rRNA gene.

Stage Name	Temperature	Time	The Number of Cycles
General denaturation	94 °C	4 min	1
Denaturation	94 °C	30 s	37
Primer annealing	53 °C	30 s
Elongation	72 °C	30 s
Final elongation	72 °C	5 min	1