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. 2022 Aug 11;14(8):547. doi: 10.3390/toxins14080547

Table 4.

Method validation for HPLC analysis for the detection of AFs.

Aflatoxin Rt (min) LOD *
(ng mL−1)
LOQ *
(ng mL−1)
Calibration Curve R2
AFB1 5.24 0.002 0.005 y = 67093x + 33.842 0.9997
AFB2 4.02 0.001 0.002 y = 45747x − 709.54 0.9995
AFG1 2.71 0.002 0.005 y = 33025x + 27.800 0.9996
AFG2 2.50 0.001 0.002 y = 63802x − 85.618 0.9991

LOD * = limit of detection. LOQ * = limits of quantification. Rt = retention time.