Figure 1.

hVECs support ZIKV replication and viral production. (A) VK2 cell was infected with ZIKV-PR at a low multiplicity infection of 0.01. At days 0, 2, 4, and 6, positive-sense viral RNA were extracted from cell lysate and subjected to qRT-PCR analysis to monitor the dynamics of the vRNA. (B) Extracellular viral release from the culture supernatant of the same infected cells as in (A) was measured using qRT-PCR. (C) PFU viral titer from the supernatant of ZIKV-infected VK2 cells at 2, 4, and 6 days post-infection were quantified and plotted. (D) Negative-sense viral RNA, which is a molecular marker for de novo viral replication, was analyzed from infected cell lysates harvested at day 6 post-infection using RT-PCR. The PCR product of negative-sense vRNA was located at 102 bp. The t test was used to measure p-values.