Table 1.
Yields and quality metrics for rooibos DNA samples from two rooibos plants generated using various DNA extraction and purification procedures.
| DNA Extraction Method | Plant | Purification Method + | 260/280 | 260/230 | Qubit (ng/µL) | Nanodrop (ng/µL) | Q:ND * | Starting Material | Total Yield (µg) | Sequencing Run |
|---|---|---|---|---|---|---|---|---|---|---|
| SDS | 1 | C:I extraction | 2.12 | 1.93 | 20.8 | 67.5 | 0.3 | 1 g leaves | 2.0 | n/a |
| CTAB | 1 | None | 2.11 | 2.01 | 358 | 1944 | 0.2 | 1 g leaves | 35.8 | 1 |
| CTAB | 1 | Zymoclean | 2.94 | 0.04 | 6.3 | 13.0 | 0.5 | 3.5 µg DNA | 0.06 | n/a |
| CTAB | 1 | Genomic-tip 1 | 2.07 | 1.91 | 102 | 1816 | 0.1 | 100 µg DNA | 2.0 | 3 |
| CTAB | 1 | Genomic-tip 2 | 2.06 | 2.22 | 347 | 1143 | 0.3 | 100 µg DNA | 6.9 | 4 |
| CTAB | 1 | DNeasy | 1.92 | 2.38 | 323 | 337 | 1.0 | 10 µg DNA | 6.4 | 2 |
| CTAB | 2 | DNeasy | 1.98 | 2.35 | 222 | 260 | 0.9 | 10 µg DNA | 4.4 | 6 |
| CTAB | 1 | DNeasy | 1.92 | 2.33 | 325 | 381 | 0.9 | 10 µg DNA | 6.5 | 7 |
| CTAB | 1 | DNeasy | 1.97 | 2.35 | 324 | 336 | 1.0 | 10 µg DNA | 6.4 | 8 |
+ Purification procedures include chloroform:isoamyl alcohol (C:I) extraction, the Zymoclean™ Large Fragment DNA Recovery Kit, the QIAGEN® DNeasy PowerClean CleanUp Kit, and the QIAGEN® Genomic-tip 500/G (Genomic-tip 1 and Genomic-tip 2). * Qubit:Nanodrop ratio.