Table 2.
Sequencing statistics for seven MinION runs with genomic DNA samples from rooibos (Quality ≥ 7).
| Run1 | Run2 | Run3 | Run4 | Run5 | Run6 | Run7 | |
|---|---|---|---|---|---|---|---|
| total gigabases (Gbp) | 1.01 | 4.56 | 6.62 | 19.08 | 11.24 | 4.26 | 2.24 |
| # reads (×1 M) | 0.30 | 1.21 | 1.56 | 4.98 | 3.91 | 4.25 | 2.30 |
| N50 length (bp) | 4372 | 6002 | 6641 | 6483 | 5642 | 1666 | 1480 |
| median length (bp) | 2656 | 2519 | 3027 | 2496 | 1607 | 569 | 587 |
| max length (bp) | 60,435 | 11,5278 | 75,142 | 79,588 | 180,920 | 116,028 | 110,672 |
| median Quality | 10 | 11.1 | 11.4 | 11.3 | 11.2 | 11.2 | 11 |
| Read length distributions: | |||||||
| >10 kb | 11,270 | 86,876 | 136,418 | 401,054 | 167,363 | 10,551 | 4616 |
| >20 kb | 598 | 13,964 | 17,790 | 54,087 | 6700 | 460 | 215 |
| >50 kb | 2 | 91 | 61 | 123 | 2 | 3 | 4 |
| >100 kb | 0 | 1 | 0 | 0 | 1 | 1 | 2 |
| >200 kb | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Top 5 longest reads (bp) and their mean quality score: | |||||||
| 1 | 60,435 (9.0) | 115,278 (10.0) | 75,142 (10.9) | 79,588 (8.5) | 180,920 (19.6) | 116,028 (7.9) | 110,672 (14.0) |
| 2 | 57,561 (8.8) | 84,582 (9.8) | 71,014 (9.0) | 75,403 (11.6) | 135,178 (7.1) | 64,587 (14.9) | 102,055 (14.7) |
| 3 | 45,472 (10.8) | 74,614 (12.7) | 69,281 (10.6) | 74,146 (13.1) | 91,826 (7.9) | 61,155 (7.7) | 99,741 (6.7) |
| 4 | 42,770 (9.6) | 71,567 (10.0) | 65,014 (11.0) | 70,447 (9.3) | 47,153 (10.2) | 44,661 (10.3) | 95,441 (14.8) |
| 5 | 42,761 (9.6) | 71,536 (11.0) | 64,036 (10.0) | 68,961 (10.1) | 46,147 (12.4) | 39,799 (9.9) | 90,125 (14.6) |
Run1: plant 1 unpurified DNA; run2: plant 1 Genomic-tip 1; run3: plant 1 Genomic-tip 2; run4: plant 1 DNeasy; run5: plant 2 DNeasy; runs 6 and 7: oxidized plant 1 DNeasy.