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. 2022 Aug 16;14(16):3357. doi: 10.3390/nu14163357

Figure 3.

Figure 3

Intermediate FPHL HFs reveal lower metabolic activity ex vivo. (a) Lactate, (b) glucose, (c) glutamine, and (d) glutamate concentration was measured in the WCM culture medium after 24 h HF culture following recommended protocols for Glo™ Assays kits (Promega®, Walldorf, Germany). Mean ± SEM recorded luminescence from n = 9−19 HFs/ group from n = 3−4 FPHL donors. Red dotted lines indicate the amount in the blank, non-conditioned, WCM culture medium. GraphPad Prism 9.0; Kruskal–Wallis test and Dunn’s multiple comparison test # p < 0.05, ### p < 0.001, #### p < 0.00001; Multiple Mann–Whitney test (Benjamini and Hochberg 5%FDR correction), * p < 0.05, **** p < 0.00001. t: terminal; i/m: intermediate/miniaturized.