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. 2022 Aug 16;14(16):3357. doi: 10.3390/nu14163357

Figure 4.

Figure 4

Intermediate parietal HFs display lower VEGF and TSP-1 expression. (a) Representative images of in situ Vascular Endothelial Growth Factor(VEGF)/Cluster of Differentiation 31 (CD31) (double immunofluorescence staining of the bulb, sub-bulge, and bulge areas of a terminal (t) hair follicle from FPHL occipital scalp skin and of a terminal, and an intermediate/miniaturized (i/m) hair follicle from FPHL parietal scalp skin. (b) VEGF protein expression levels were measured by quantitative (immuno-)histomorphometry in the connective tissue sheath (CTS) of hair follicle bulb, sub-bulge, and bulge regions, and in the dermal papilla (DP) of intermediate/miniaturized (i/m) and/or terminal (t) hair follicles from n = 4–5 FPHL donors (1: red, 2: green, 3: blue, 4: purple, 5: pink). Mean ± SEM from 2–34 hair follicles/group. GraphPad 9.0. Kruskal–Wallis with Dunn’s multiple comparison ## p < 0.01; Multiple Unpaired t-test or Mann Whitney test (Original FDR method -Benjamini and Hochberg −5%FDR correction), *** p < 0.001. (c) Representative images of in situ TSP-1/CD31 double immunofluorescence staining of bulb, sub-bulge, and bulge areas of a terminal hair follicle from FPHL occipital scalp skin and of a terminal, and an intermediate/miniaturized hair follicle from FPHL parietal scalp skin. (d) TSP-1 protein expression was measured by quantitative (immuno-)histomorphometry in the connective tissue sheath (CTS) of hair follicle bulb, and bulge and in the hair follicle epithelium at the sub-bulge and bulge level of intermediate/miniaturized (i/m) and/or terminal (t) hair follicles from n = 4–5 FPHL donors (1: red, 2: green, 3: blue, 4: purple, 5: pink). Mean ± SEM from 8–27 hair follicles/group. GraphPad 9.0. (CTS) Ordinary one-way Anova with Tukey’s multiple comparison test # p < 0.05; Multiple Unpaired t-test (Original FDR method -Benjamini and Hochberg −5%FDR correction), * p < 0.05; (epithelium) Kruskal-Wallis with Dunn’s multiple comparison, not significant (n.s.); multiple Mann Whitney test (Original FDR method -Benjamini and Hochberg −5%FDR correction), n.s.