Table 1.

Different levels of factors and their responses as low, medium, and high in optimization of β–sito–Alg/Ch/NPs in Box Behnken design.

Factors	Levels Used
	Low (−1)	Medium (0)	High (+1)
X1: Chitosan, %w/v	0.15	0.22	0.30
X2: Sodium alginate, %w/v	0.25	0.38	0.50
X3: Calcium chloride, mM	15	22.5	30
Responses
Y1: Particle size, nm		Minimize
Y2: PDI		Minimize
Y3: Entrapment efficiency, %		Maximize