Table 2.

The results of in vitro assessed parameter of formulations of Run (1–17) for responses in optimization process of β–sito–Alg/Ch/NPs.

Runs	Independent Variables			Responses
	X1, %w/v	X2, %w/v	X3, mM	Y1, nm	Y2	Y3, %
* 1	0.15	0.38	15.00	21.00	0.120	77.00
2	0.22	0.50	30.00	42.00	0.310	78.00
3	0.30	0.38	30.00	40.00	0.61	91.00
* 4	0.22	0.25	15.00	45.00	0.204	72.00
5	0.22	0.38	22.00	34.00	0.380	75.00
* 6	0.22	0.38	22.00	34.90	0.420	76.00
7	0.30	0.50	22.00	31.00	0.570	89.00
8	0.30	0.38	15.00	42.00	0.510	86.00
9	0.22	0.25	30.00	43.00	0.530	76.00
10	0.15	0.25	22.50	23.00	0.260	73.00
* 11	0.15	0.50	22.50	16.00	0.330	79.00
12	0.22	0.38	22.50	32.00	0.340	78.00
13	0.22	0.38	22.50	33.00	0.360	76.30
* 14	0.22	0.38	22.50	33.60	0.402	77.00
15	0.22	0.50	15.00	35.00	0.310	76.00
16	0.30	0.25	22.00	38.00	0.730	88.00
17	0.15	0.38	30.00	27.00	0.350	77.60

X1: Chitosan, %w/v, X2: Sodium alginate, %w/v, X3: Calcium chloride (mM), Y1: Particle size (nm), Y2: PDI, Y3: % Entrapment efficiency. * Replicas.