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. 2022 Aug 22;27(16):5345. doi: 10.3390/molecules27165345

Figure 4.

Figure 4

Purification and characterization of the recombinant protein AsCDA from Acinetobacter schindleri MCDA01: (A) heterologous expression and purification of AsCDA were analyzed by SDS-PAGE on a 12% gel after Coomassie brilliant blue R-250 staining; (B) specific activity of purified AsCDA to colloidal chitin, α-chitin, and β-chitin. Lane M, proteins marker with standard molecular Masses; lane 1, negative control (empty vector pET-28a); lane 2, Fermentation supernatant from Escherichia coli BL21 containing expression vector pET28a-AsCDA induced with IPTG; lane 3, insoluble phase of cellular extracts from Escherichia coli BL21 containing expression vector pET28a-AsCDA induced with IPTG; lane 4, soluble phase of cellular extracts from Escherichia coli BL21 containing expression vector pET28a-AsCDA induced with IPTG; lane 5, effluent fractions when all proteins were washed clean with elution 80 mM imidazole conc; lane 6, purified AsCDA with elution 300 mM imidazole.