Table 1.

Process parameters used for the formulation assessment and the production of immunomodulating nanovaccines with the probe sonication method.

Probe Sonication
Exp. No.	API	DP Solution	DP Volume (mL)	CP Solution	CP Volume (mL)	Total Sonication Time (min)	EP Solution	EP Volume (mL)
1	Placebo	5 wt% PLGA, 95 wt% DCM	3	2 wt% PVA	9	2	MilliQ water	294
2	IMM60 and peptide 1 or peptide 2 or peptide 3	3.9 wt% PLGA, 74.3 wt% DCM, 0.006 wt% IMM60, 0.04 wt% NY-ESO-01, 21.7 wt% DMSO	4.06	2 wt% PVA	12.18	2	MilliQ water	290
3	Placebo	3.9 wt% PLGA, 74.4 wt% DCM, 21.7 wt% DMSO	4.06	2 wt% PVA	12.18	2	MilliQ water	290