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The expression of gE in mammalian cells was tested by transducing HepG2 cells with rBV Bac–gE (carrying P10 only or P10 and CMV promotors) at different multiplicity of infection (MOI). Red fluorescence suggests the expression of gE in HepG2 cells; blue fluorescence (DAPI) indicates the location of the nucleus (bar = 50 μm). Four technical replicates were performed during one experiment.
