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(A) Scheme of the DNA region around the pyc gene in wild-type S. clavuligerus 27064 (a), disrupted mutant S. clavuligerus pyc::aph-a (b), and replacement mutant S. clavuligerus pyc::aph-b (c). (B) Hybridization of EcoRI-NcoI-digested DNA with the 1.3-kb BamHI probe containing the aph gene. Lanes: 1, S. clavuligerus pyc::aph-a; 2, S. clavuligerus 27064, 3 and 4, S. clavuligerus pyc::aph-b; 5, size marker (λ phage digested with PstI).
