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. 2022 Aug 17;14(8):1801. doi: 10.3390/v14081801

Table 2.

Gilt anti-PEDV IgG and IgA response in serum as tested by PEDV whole virus-based ELISA (S/P ratios shown in the table).

Antibody Isotype Group Gilt ID DP1V 0 DP1V 7 DP1V 14 DP2V 0 DP2V 7 Farrow DPC 0 DPC 7 DPC 16
D0 D7 D14 D21 D28 D36–D39 D42 D49 D58 *
IgG T01 5003 0.5411 0.6365 0.6584 0.5189 0.0329 0.0209 0.0269 0.2749 2.3297
V/C 5026 0.1256 0.1683 0.1663 0.0867 0.0886 1.3576 3.1444 2.9761 3.2600
5046 0.3237 0.4771 0.4602 0.3187 0.1614 0.3675 0.6325 2.9622 3.1275
6174 0.3353 0.5777 0.7291 0.4890 0.3546 0.2918 0.2231 3.3088 3.4014
Average 0.3314 0.4649 0.5035 0.3533 0.1594 0.5095 1.0067 a 2.3805 a 3.0297 a
T02 5043 0.3836 0.7440 0.6580 0.6454 0.1237 0.1111 0.0435 0.3884 2.8000
N/C 5048 0.3092 0.5604 0.4734 0.4715 0.2415 0.2135 0.1188 1.0966 2.8464
6173 0.5662 0.6618 0.5894 0.5710 0.2435 0.1585 0.2628 0.8058 2.9111
5044 0.5507 0.5130 0.3150 0.4280 0.1208 0.1903 0.1575 1.0763 2.3353
Average 0.4524 0.6198 0.5090 0.5290 0.1824 0.1684 0.1457 a 0.8418 a 2.7232 a
T03 6175 0.8609 0.6300 0.8213 0.6106 0.1246 0.0918 0.0986 0.1063 0.0928
N/N 5042 0.9594 0.6174 0.9488 0.5758 0.1266 0.0454 0.0879 0.0551 0.1913
Average 0.9102 0.6237 0.8851 0.5932 0.1256 0.0686 0.0933 a 0.0807 a 0.1421 b
IgA T01 5003 0.0949 0.1227 0.1031 0.1332 0.0796 0.0561 0.0326 0.4060 4.4883
V/C 5026 0.0437 0.0757 0.1018 0.0679 0.1906 0.8251 2.7624 2.6057 3.6632
5046 0.1436 0.1384 0.1410 0.0731 0.1214 0.5718 0.2154 1.5953 2.4047
6174 0.0424 0.0509 0.0809 0.0666 0.0731 0.0692 0.0313 0.2415 1.6893
Average 0.0812 0.0969 0.1067 0.0852 0.1162 0.3806 0.7604 a 1.2121 a 3.0614 a
T02 5043 0.0587 0.1174 0.0861 0.1061 0.0387 0.0687 0.0225 0.3870 3.3620
N/C 5048 0.0300 0.0462 0.0350 0.0474 0.0424 0.0474 0.0300 0.1598 2.7790
6173 0.1099 0.0899 0.0662 0.0999 0.1785 0.1211 0.1461 0.3034 2.7665
5044 0.0762 0.0624 0.0400 0.0787 0.0824 0.1548 0.0924 1.3408 3.6554
Average 0.0687 0.0790 0.0568 0.0830 0.0855 0.0980 0.0728a 0.5478 a 3.1407 a
T03 6175 0.3908 0.2709 0.4370 0.4295 0.1473 0.4732 0.2222 0.1461 0.0899
N/N 5042 0.1523 0.0737 0.1673 0.0861 0.1273 0.0300 0.1248 0.0375 0.0512
Average 0.2716 0.1723 0.3022 0.2578 0.1373 0.2516 0.1735 a 0.0918 a 0.0706 b

Notes: V/C: vaccination/challenge; N/C: non-vaccination/challenge; N/N: non-vaccination/non-challenge. DP1V: day post 1st vaccination; DP2V: day post 2nd vaccination; DPC: day post challenge; D0-D58: day of study. * Gilts euthanized on D58. Different letters (a and b) indicate statistically significant difference of IgG between groups and IgA between groups at each time point D42, D49 and D58. The positive cutoff for serum IgG and IgA was an S/P ratio equal to or greater than 0.7, as indicated in red.