Table 2.
Antibody Isotype | Group | Gilt ID | DP1V 0 | DP1V 7 | DP1V 14 | DP2V 0 | DP2V 7 | Farrow | DPC 0 | DPC 7 | DPC 16 |
---|---|---|---|---|---|---|---|---|---|---|---|
D0 | D7 | D14 | D21 | D28 | D36–D39 | D42 | D49 | D58 * | |||
IgG | T01 | 5003 | 0.5411 | 0.6365 | 0.6584 | 0.5189 | 0.0329 | 0.0209 | 0.0269 | 0.2749 | 2.3297 |
V/C | 5026 | 0.1256 | 0.1683 | 0.1663 | 0.0867 | 0.0886 | 1.3576 | 3.1444 | 2.9761 | 3.2600 | |
5046 | 0.3237 | 0.4771 | 0.4602 | 0.3187 | 0.1614 | 0.3675 | 0.6325 | 2.9622 | 3.1275 | ||
6174 | 0.3353 | 0.5777 | 0.7291 | 0.4890 | 0.3546 | 0.2918 | 0.2231 | 3.3088 | 3.4014 | ||
Average | 0.3314 | 0.4649 | 0.5035 | 0.3533 | 0.1594 | 0.5095 | 1.0067 a | 2.3805 a | 3.0297 a | ||
T02 | 5043 | 0.3836 | 0.7440 | 0.6580 | 0.6454 | 0.1237 | 0.1111 | 0.0435 | 0.3884 | 2.8000 | |
N/C | 5048 | 0.3092 | 0.5604 | 0.4734 | 0.4715 | 0.2415 | 0.2135 | 0.1188 | 1.0966 | 2.8464 | |
6173 | 0.5662 | 0.6618 | 0.5894 | 0.5710 | 0.2435 | 0.1585 | 0.2628 | 0.8058 | 2.9111 | ||
5044 | 0.5507 | 0.5130 | 0.3150 | 0.4280 | 0.1208 | 0.1903 | 0.1575 | 1.0763 | 2.3353 | ||
Average | 0.4524 | 0.6198 | 0.5090 | 0.5290 | 0.1824 | 0.1684 | 0.1457 a | 0.8418 a | 2.7232 a | ||
T03 | 6175 | 0.8609 | 0.6300 | 0.8213 | 0.6106 | 0.1246 | 0.0918 | 0.0986 | 0.1063 | 0.0928 | |
N/N | 5042 | 0.9594 | 0.6174 | 0.9488 | 0.5758 | 0.1266 | 0.0454 | 0.0879 | 0.0551 | 0.1913 | |
Average | 0.9102 | 0.6237 | 0.8851 | 0.5932 | 0.1256 | 0.0686 | 0.0933 a | 0.0807 a | 0.1421 b | ||
IgA | T01 | 5003 | 0.0949 | 0.1227 | 0.1031 | 0.1332 | 0.0796 | 0.0561 | 0.0326 | 0.4060 | 4.4883 |
V/C | 5026 | 0.0437 | 0.0757 | 0.1018 | 0.0679 | 0.1906 | 0.8251 | 2.7624 | 2.6057 | 3.6632 | |
5046 | 0.1436 | 0.1384 | 0.1410 | 0.0731 | 0.1214 | 0.5718 | 0.2154 | 1.5953 | 2.4047 | ||
6174 | 0.0424 | 0.0509 | 0.0809 | 0.0666 | 0.0731 | 0.0692 | 0.0313 | 0.2415 | 1.6893 | ||
Average | 0.0812 | 0.0969 | 0.1067 | 0.0852 | 0.1162 | 0.3806 | 0.7604 a | 1.2121 a | 3.0614 a | ||
T02 | 5043 | 0.0587 | 0.1174 | 0.0861 | 0.1061 | 0.0387 | 0.0687 | 0.0225 | 0.3870 | 3.3620 | |
N/C | 5048 | 0.0300 | 0.0462 | 0.0350 | 0.0474 | 0.0424 | 0.0474 | 0.0300 | 0.1598 | 2.7790 | |
6173 | 0.1099 | 0.0899 | 0.0662 | 0.0999 | 0.1785 | 0.1211 | 0.1461 | 0.3034 | 2.7665 | ||
5044 | 0.0762 | 0.0624 | 0.0400 | 0.0787 | 0.0824 | 0.1548 | 0.0924 | 1.3408 | 3.6554 | ||
Average | 0.0687 | 0.0790 | 0.0568 | 0.0830 | 0.0855 | 0.0980 | 0.0728a | 0.5478 a | 3.1407 a | ||
T03 | 6175 | 0.3908 | 0.2709 | 0.4370 | 0.4295 | 0.1473 | 0.4732 | 0.2222 | 0.1461 | 0.0899 | |
N/N | 5042 | 0.1523 | 0.0737 | 0.1673 | 0.0861 | 0.1273 | 0.0300 | 0.1248 | 0.0375 | 0.0512 | |
Average | 0.2716 | 0.1723 | 0.3022 | 0.2578 | 0.1373 | 0.2516 | 0.1735 a | 0.0918 a | 0.0706 b |
Notes: V/C: vaccination/challenge; N/C: non-vaccination/challenge; N/N: non-vaccination/non-challenge. DP1V: day post 1st vaccination; DP2V: day post 2nd vaccination; DPC: day post challenge; D0-D58: day of study. * Gilts euthanized on D58. Different letters (a and b) indicate statistically significant difference of IgG between groups and IgA between groups at each time point D42, D49 and D58. The positive cutoff for serum IgG and IgA was an S/P ratio equal to or greater than 0.7, as indicated in red.