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. 2022 Aug 17;14(8):1801. doi: 10.3390/v14081801

Table 3.

Gilt anti-PEDV IgG and IgA response in colostrum/milk as tested by PEDV whole virus-based ELISA (S/P ratios shown in the table).

Antibody Isotype Group Gilt ID D42 D44 D46 D49 D52 D56
DPC 0 DPC 2 DPC 4 DPC 7 DPC 10 DPC 14
IgG T01 V/C 5003 0.7903 0.5831 X 0.0166 0.8213 1.1731
5026 0.0346 0.9321 1.1440 0.6856 0.6884 0.7091
5046 0.0596 0.0568 0.0305 0.8089 1.5623 1.1413
6174 0.0014 0.0055 0.2008 0.4474 3.2729 3.6745
Average 0.2215 a 0.3944 a 0.4584 a 0.4896 a 1.5862 a 1.6745 a
T02 N/C 5043 −0.0194 0.0097 0.0222 0.0443 0.4437 0.6971
5048 0.0055 0.0180 −0.0069 0.1773 2.2995 2.8063
6173 −0.0235 0.0097 0.0360 0.1316 0.8698 1.1515
5044 0.0235 0.0014 0.0222 0.1510 X X
Average −0.0035 a 0.0097 a 0.0184 a 0.1261 a 1.2043 a 1.5516 a
T03 N/N 6175 0.0402 0.0000 0.0499 0.0360 0.0147 −0.0065
5042 0.0055 −0.0028 0.0568 0.0817 0.0147 0.0374
Average 0.0229 a −0.0014 a 0.0534 a 0.0589 a 0.0147 a 0.0155 a
IgA T01 V/C 5003 0.4527 0.3953 X 0.0721 0.1840 0.7342
5026 0.1324 0.5735 0.6573 0.6446 0.8345 1.1091
5046 0.1110 0.4197 0.2425 0.3359 0.8617 0.8111
6174 0.0039 0.0662 0.1821 0.1071 0.1918 0.5034
Average 0.1750 a 0.3637 a 0.3606 a 0.2899 a 0.5180 a 0.7895 a
T02 N/C 5043 −0.0068 0.0302 0.0876 0.0380 0.1255 0.4649
5048 −0.0049 0.0292 0.0263 0.1130 0.3385 0.8157
6173 0.0010 0.1188 0.1402 0.3359 0.2592 0.6852
5044 0.0156 0.0146 0.0769 0.0808 X X
Average 0.0012 a 0.0482 b 0.0828 a 0.1419 a 0.2411 a 0.6553 a
T03 N/N 6175 0.0516 0.0166 0.0331 0.0380 0.0148 0.0033
5042 0.0380 0.0282 0.1061 0.0935 0.0618 0.0493
Average 0.0448 a 0.0224 b 0.0696 a 0.0658 a 0.0383 a 0.0263 b

Note: V/C: vaccination/challenge; N/C: non-vaccination/challenge; N/N: non-vaccination/non-challenge. DP1V: day post 1st vaccination; DP2V: day post 2nd vaccination; DPC: day post challenge; D0-D58: day of study. X: milk samples not available. Different letters (a and b) indicate statistically significant difference of IgG between groups and IgA between groups at each time point. The positive cutoff for colostrum IgG and IgA was an S/P ratio equal or greater than 0.6, as indicated in red.