Skip to main content
. 2022 Aug 12;15:911476. doi: 10.3389/fnmol.2022.911476

FIGURE 1.

FIGURE 1

Diagram of experimental timeline and hypothesis of this study. (A) Experimental timelines. EM, electron microscopy; i.t, intrathecal; ITC, intra-thalamic collagenase IV injection; ITS, intra-thalamic saline injection; MEA, multi-electrode array; TUNEL, terminal deoxynucleotidyl transferase-mediated nick end labeling; (B) time courses of bilateral mechanical pain hypersensitivity induced by left ITC with ITS as control. Contrl, contralateral; Ipsil, ipsilateral; PWMT, paw withdrawal mechanical threshold; ***p < 0.001 Day 3, Day 7, Day 10 vs. one day prior to surgery; ###p < 0.001 ITC-Ipsil vs. ITS-Ipsil; ***p < 0.001 ITC-Contrl vs. ITS-Contrl; n = 44 rats for ITS, n = 59 rats for ITC. (C) Hypothetic scheme proposes that post-thalamic hemorrhagic stroke (THS) pain hypersensitivity should be mediated by the THS-induced secondary neural damage and neuroinflammation in the spinal dorsal horn through retrograde axonal degeneration.