FIGURE 2.

Extracellular MEA mapping of spinal dorsal horn neurons 7 days after ITS or ITC. (A) Photomicrograph showing tetrode track and MEA recording sites at the spinal dorsal horn labeled with Iba1 and DAPI. (B) Waveforms and spike traces of five units sorted out by one tetrode (0–3). (C) Identification of three classes of dorsal horn neurons (LTM, WDR, and NS) by electrophysiological properties to brush, pressure, and pinch stimuli applied to their corresponding peripheral receptive fields in one hind paw pad showing by peri-stimulus time histograms and raster displays. (D) Spatial localization and counts of three classes of units across the dorsal horn from the surface of the cord dorsum to the deep layers by 60- to 80-μm step advance following ITS (n = 5) and ITC (n = 5), respectively.