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. 2022 Aug 12;15:911476. doi: 10.3389/fnmol.2022.911476

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Copyright © 2022 Liang, Chen, Yang, Yang, Yu, Yang, Wang, Wang, Sun and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Retrograde degeneration induced neuronal cell apoptosis of spinal dorsal horn caused by ITC-induced thalamic hemorrhagic stroke. Representative immunofluorescent photomicrographs showing co-localization of NeuN with Bcl-2 (A) and with Bax (C) in the spinal dorsal horn 7 days after ITC. Quantification of the mean immunofluorescent intensity of Bcl-2 (B) and Bax (D). ***p < 0.001 ITC vs. ITS, n = 3 rats/group. (E) Representative fluorescent photomicrographs of TUNEL- and DAPI-labeled cells. (F) Quantitative data of TUNEL-labeled cells. ***p < 0.001 ITC vs. ITS, n = 3 rats/group. (G) and (H) Western blot assay and quantitative analysis of Bcl-2 (G) and Bax (H) protein expressions in bilateral dorsal cord following 7 days after ITC or ITS. *p < 0.05, **p < 0.01, ITC vs. ITS, n = 5 rats/group.