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. 2022 Aug 10;14(8):1665. doi: 10.3390/pharmaceutics14081665

Figure 5.

Figure 5

17-AAG increased the expression of claudin 5 in cortex and PAG after induction of CSD. Female Sprague Dawley rats underwent dural cannulation surgery. After recovery, 17-AAG (0.5 nmol) or vehicle (1%DMSO in saline) were injected via dural cannula 24 h before cortical KCl injection. Cortex and PAG tissue were harvested 90 min after CSD induction and subjected to Western immunoblotting. Representative images of cortex (A) and PAG (B) samples showing the expression of claudin 5 and α-tubulin, as loading control. Pretreatment of 17-AAG increased the expression of claudin 5 in cortex (A) and PAG (B) samples as compared to vehicle control. All data in panel A represent the % of vehicle-treated relative expression ± SEM (n = 4 in each group), whereas in panel B, all data represent the % of vehicle-aCSF pretreated controls’ relative expression ± SEM (n = 4/condition). * p < 0.05 as assessed by unpaired t-test. Circles and squares indicate individual subjects.