Table 1.
Gene expression profile in the kidney of KLF11+/+ and KLF11−/− mice
| Gene | Sham | IRI | ||
|---|---|---|---|---|
| KLF11+/+ | KLF11−/− | KLF11+/+ | KLF11−/− | |
| KLF11 | 4.01±0.53 | ND | 4.82±0.35 | ND |
| MCP-1 | 2.47±0.2 | 2.23±0.17 | 17.49±1.56 | 26.11±2.72a |
| PAI-1 | 1.33±0.15 | 1.34±0.08 | 24.15±4.27 | 70.87±12.24b |
| SMAD3 | 6.14±0.31 | 5.87±0.49 | 9.22±0.6 | 12.37±0.62c |
| TNF-α | 3.07±0.42 | 2.12±0.17 | 16.63±3.29 | 15.36±2.09 |
| TGF-β1 | 4.7±0.31 | 4.06±0.22 | 13.51±1.23 | 16.16±1.04 |
| IL-1β | 1.07±0.12 | 1.22±0.26 | 4.25±0.83 | 4.75±0.81 |
| ICAM1 | 6.48±0.3 | 7.03±0.45 | 16.76±1.18 | 16.43±0.67 |
| MMP-9 | 1.5±0.2 | 1.41±0.2 | 11.19±3.26 | 17.2±2.19 |
| PGC-1α | 18.66±0.79 | 18.05±0.41 | 9.18±0.63 | 7.68±0.24a |
Quantitative real-time RT-PCR analysis was performed. Shown is mRNA expression of multiple genes in the kidney 1 day after IRI for 22 minutes or sham IRI. Values are the mean±SEM; n=5 for each sham group, and n=6 and 7 for KLF11+/+ and KLF11−/− IRI groups, respectively. Relative quantification was performed against a standard curve constructed for each mRNA target, normalized for expression of 18S rRNA, and expressed in arbitrary units. A t test was used for parametric data, and the Mann–Whitney U test was used for nonparametric data. KLF, Krüppel-like factor; ND, not detected; IRI, ischemia-reperfusion injury.
aP<0.05, bP<0.01, cP<0.005, KLF11−/− IRI versus KLF11+/+ IRI.