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. 2022 Jul 22;10:936584. doi: 10.3389/fbioe.2022.936584

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Copyright © 2022 An, Koh, Ahn, Kim, Han, Lee, Kim and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of APM gene expression and HLA class I molecule surface presentation by transcription factors. (A–H) Relative mRNA levels in non-transfected control (NTC), IFN-γ-treated (IFN-γ), RelA-transfected (RelA), NLRC5-transfected (NLRC5), and constitutive mutant form of NLRC5-transfected (Nmut) NSCs (n = 4). (I) Representative flow cytometry plot of surface HLA-BC presentation. (J,K) Percentage HLA-BC-positive populations (J) and MFI values compared to NTCs (K), (n = 4). (L) Immunocytochemistry of β2m surface presentation in response to transcription factors. Magnified image shows β2m on the cell surface; all images are fluorescence micrographs. Scale bar, 50 μm. Data are means ± SEMs.