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Apparent kinetic parameters for the CMP-Neu5Ac hydrolysis activities of Psp2,6ST(15–501)-His6 and A235D mutant.
| Enzymes | Km (mM) | kcat (min−1) | kcat/Km (mM−1 min−1) |
|---|---|---|---|
| His6-Pd2,6ST(16–497) | (1.8±0.4)×10 | (2.2±0.2)×102 | 1.2×10 |
| Psp2,6ST(15–501)-His6 | (1.7±0.4)×10 | (2.9±0.3)×102 | 1.7×10 |
| Psp2,6ST(15–501)-His6 A235D mutant | (3.8±0.5)×10 | (2.5±0.2)×102 | 6.6 |
