Comparison of the kinetic parameters of Au@Pt and Au@PtRu nanorods for the oxidation of TMB by H₂O₂^a.

Catalyst	[E] μM	Substrate	V max (M s−1)	K m (mM)	k cat (s−1)	k cat/Km (s−1 mM−1)
Au@PtRu	32.5 × 10−6	TMB	1.3 × 10−6	0.7	3.4 × 105	4.9 × 105
		H2O2	1.5 × 10−6	0.23 × 103	4.0 × 105	17.4 × 102
Au@Pt	32.5 × 10−6	TMB	5.5 × 10−7	0.45	1.5 × 105	3.3 × 105
		H2O2	1.4 × 10−6	1.2 × 103	3.7 × 105	3.1 × 102

^a[E] is the concentration of nanozyme. The particle number of Au@PtRu or Au@Pt nanozyme is calculated using the density and diameter of the Au-rod core. Dividing the particle number by the Avogadro constant gives the molar concentration of the nanozyme. K_m: Michaelis constant; V_max: maximal reaction velocity; k_cat: catalytic constant; k_cat/K_m: catalytic efficiency.