Skip to main content
. 2022 Aug 26;17(8):e0273013. doi: 10.1371/journal.pone.0273013

Fig 7. Neighbour-joining tree generated from concatenated sequences of the seven housekeeping genes of all the 16 STs identified in the present study.

Fig 7

The novel STs are marked with asterisks (*). ST1, ST2, ST11, ST32, ST47, ST123, ST41, and ST67 (●) were added for comparison purposes. Software: The Molecular Evolution and Genetic Analysis 7.0.26 (MEGA7). B) eBURST analysis of Clostridioides difficile using STs available in the MLST database in December 2021. Each ST is represented by a circle, and lines connect single-locus variants. Khaki circles represent predicted founders. Detail showing the relation of four out of five STs from clade 2 detected in the present study (black arrows), including three Brazilian STs (462, 463, and 464). The remaining new sequence type (ST869) was a singleton.