The novel STs are marked with asterisks (*). ST1, ST2, ST11, ST32, ST47, ST123, ST41, and ST67 (●) were added for comparison purposes. Software: The Molecular Evolution and Genetic Analysis 7.0.26 (MEGA7). B) eBURST analysis of Clostridioides difficile using STs available in the MLST database in December 2021. Each ST is represented by a circle, and lines connect single-locus variants. Khaki circles represent predicted founders. Detail showing the relation of four out of five STs from clade 2 detected in the present study (black arrows), including three Brazilian STs (462, 463, and 464). The remaining new sequence type (ST869) was a singleton.