FIG. 3.

Transcriptional analysis of some act genes in the recombinant strains carrying the actIII-actI region in high copy number. Transcription was determined for the actI-ORF1 (A), actVI-ORFA (B), and actVI-ORF1 (C) genes. The RNAs were extracted from the following S. coelicolor strains: J1501 carrying pIJ486 as a control (lane 1); J1501 carrying pMF1135, the actIII-actI intergenic region, in high copy number (lane 2); the same strain carrying, in addition to pMF1135, the wild type actII-ORF4 gene (pPAS4) (lane 3) or the actII-ORF4-177 gene (pPAS1) (lane 4); J1501 (lane 5); and E. coli tRNA (lane 6) as a control. The probes were as in Fig. 2 and were labeled at the restriction site indicated by asterisks. The sizes of the protected fragments are indicated. The size markers are as in Fig. 1.