FIG. 4.
Gel mobility shift analysis of the actVI-ORF1–ORFA intergenic region with crude extracts from Streptomyces. Lanes: 1, probe actVI-ORF1–ORFA intergenic region (329 bp); 2 and 4, cell extract and 60% ammonium sulfate precipitated from S. coelicolor PM218(pIJ702), respectively; 3 and 5, cell extract and 60% ammonium sulfate fraction from S. coelicolor PM218(pMF1125), respectively.