FIG. 5.

Purification pattern of the wild-type His-tagged ActII-ORF4 protein. Lanes: 1 and 2, total-cell lysate of E. coli BL21(DE3)pLysS/pET-19b not induced and induced, respectively; 3 and 4, total-cell lysate of E. coli BL21(DE3)pLysS/pPAC10 not induced and induced, respectively; 5 and 6, soluble and insoluble fractions, respectively, from a cell extract of E. coli BL21(DE3)pLysS/pPAC10; 7, fraction from a Ni²⁺ column eluted with 1 M imidazole; 8, fraction eluted with 0.1 M imidazole plus 5 mM ATP; 9, purified His-tagged ActII-ORF4 protein. Molecular masses are indicated in kilodaltons.