FIG. 7.

DNase I footprinting analysis in the actVI region. (A) Upper strand: F139 fragment 5′-labeled (G1 primer) as described in Materials and Methods. (B) Lower strand: F139 fragment 5′-labeled (G39 primer). Lanes: 1, probes; 2, wild-type His-tagged ActII-ORF4 protein (1.5 μg); 3, His-tagged ActII-ORF4-177 protein (1.5 μg). Sequencing reactions (ACGT) generated with the corresponding primers (G1 or G39) were run in parallel. The hypersensitive regions are indicated by arrows. The −10 and −35 regions of the promoters are indicated.