Table 1. Selected primer pairs for the multiplex one-step RT-PCR assay.

Primer name	Sequences (5’→3’)	Position1	Target	Amplicon (bp)	Reference
RG_Fw1_RNA1	GACTCAGATCCAGCGGGAA	246–264a	RGNNV-RNA1	169	This study
RG_Rev6_RNA1	TCCAACCTCACGGGGTGAT	396–414a
RG_SPCF1b_RNA2	CAATCGTCGGCGTAGTAATC	107–126a	RGNNV-RNA2	647	This study
RG_SPCFRev3b_RNA2	AGGAGGATGGACTTGAAGTC	735–754a
SJ_F2_RNA2	ATTACTACCCAGGCGCCAC	691–709b	SJNNV-RNA2	350	This study
R3	CGAGTCAACACGGGTGAAGA	1022–1041b			[30]

^a The position of primers is based on the sequences of strain SGWak97 (GenBank accession numbers for RNA1 AY324869 and RNA2 AY324870).

^b The position of primers is based on the sequences of strain SJNNV (GenBank accession number for RNA2 AB056572).